get rid of the pussified stock clutch diaphragm spring and get the screamin eagle one. Several years back (06? 07???) HD made the clutch "easier" to pull by reducing the spring pressure. Any time you go stage one (or even just a better tune), often the soft clutch spring will allow slippage.
I know you said you only changed fluids so imho its likely an adjustment issue as others have mentioned. I just hate the stock springs.
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